Detection of erythrocyte binding IgM and IgG by flow cytometry in sick dogs with Babesia canis canis or Babesia canis vogeli infection.

 

pubblicazione di Laboratorio d'Analisi veterinarie San Marco | del 02/03/2009

Carli E, Tasca S, Trotta M, Furlanello T, Caldin M, Solano-Gallego L. Detection of erythrocyte binding IgM and IgG by flow cytometry in sick dogs with Babesia canis canis or Babesia canis vogeli infection. Vet Parasitol. 2009 May 26;162(1-2):51-7. doi: 10.1016/j.vetpar.2009.02.002

Abstract

The aim of this study was to examine by means of flow cytometry immunophenotyping (FCI) if sick dogs infected with Babesia canis canis (B. c. canis) or Babesia canis vogeli (B. c. vogeli) had anti-erythrocyte membrane binding IgG and/or IgM at the time of diagnosis. Diagnosis of Babesia infection was assessed by blood smear and by PCR-restriction fragment length polymorphism analysis in 30 sick dogs. Signalment, clinical history, physical examination and laboratory tests of B. c. canis (n=24) and of B. c. vogeli (n=6) infected dogs were studied. The majority of B. c. canis infected dogs showed anemia (92%) predominantly non-regenerative (94%), while the B. c. vogeli infected dogs had a regenerative anemia (67%). Eccentrocytosis was present in 33% of the B. c. canis infections. Four of six B. c. vogeli infected dogs had erythrocytes membrane antibodies. One dog resulted uncertain and one resulted negative to FCI. In contrast, all the B. c. canis infected dogs were negative for erythrocytes membrane binding immunoglobulins detection. In addition, the mean percentages of erythrocytes binding IgG and IgM were statistically much lower in B. c. canis than in B. c. vogeli infected dogs. At the time of the diagnosis, the formation of erythrocyte membrane binding IgG and IgM by immune mechanisms appears not to be involved in B. c. canis infections while it is present in the majority of B. c. vogeli infections.

 

EVENTI & NEWS in evidenza

EVENTI & NEWS in evidenza

 CONTATTI

CLINICA VETERINARIA e
 PRONTO SOCCORSO

per informazioni

  • Tel

    049 85 610 98

per appuntamenti o comunicazioni alla segreteria

LABORATORIO D'ANALISI

(riservato ai medici veterinari)

AMMINISTRAZIONE

SPORTELLO PER I MEDICI VETERINARI UTENTI DEL LABORATORIO

Vi invitiamo a utilizzare questo indirizzo anche per suggerimenti ed eventuali reclami relativi all'attività del Laboratorio

AGGIORNAMENTO PER I COLLEGHI – EVENTI SAN MARCO

 DOVE SIAMO

La Clinica/Lab Veterinaria Privata S. Marco è in Via dell'Industria 3 35030 Veggiano (Padova)

Tel. 24 h - 0498561098

Ci trovate lungo la statale Padova-Vicenza, nel comune di Veggiano (tra Mestrino e Grisignano di Zocco), a pochi chilometri dal centro di Padova e dal centro di Vicenza.
Siamo facilmente raggiungibili con l’autostrada A4, a 2Km dall’uscita di Grisignano di Zocco, in direzione Padova.

TORNA SU