Stability of biomarkers of oxidative stress in canine serum.

 

pubblicazione di Laboratorio | del

Send to

Res Vet Sci. 2018 Dec;121:85-93. doi: 10.1016/j.rvsc.2018.09.007. Epub 2018 Sep 28.

Stability of biomarkers of oxidative stress in canine serum.

Rubio CP1, Tvarijonaviciute A1, Caldin M2, Hernández-Ruiz J3, Cerón JJ1, Martínez-Subiela S4, Tecles F1.

Author information

Abstract

The purpose of this study was to determine the effect of storage time and temperature on the measured concentrations of a profile of biomarkers of oxidative stress in canine serum: Trolox equivalent antioxidant capacity (TEAC) measured by two different methods, using acidic medium (TEACA), and horseradish peroxidase (TEACH), cupric reducing antioxidant capacity (CUPRAC), ferric reducing ability of plasma (FRAP), total thiol, Paraoxonase 1 (PON1), total oxidant status (TOS), ferrous oxidation xylenol orange (FOX), advanced oxidation protein products (AOPP), reactive oxygen species (ROS), and thiobarbituric reactive species (TBARS). Six serum pools from healthy dogs were divided in various aliquots and stored at 4 °C and 25 °C for a period of 6, 24 and 72 h, and at -20 °C and - 80 °C for a period of 7, 14, 30, 60, 180, and 360 days. In addition, the effect of repeated freeze-thaw cycle was evaluated. For the short-term storage of biomarkers of oxidative stress in canine serum, 4 °C is preferred to room temperature. In case of long-term storage, the optimal stability was achieved when samples were stored at -80 °C. Most of the biomarkers were affected after two freeze-thaw cycles. In addition, as individual oxidative biomarkers can show differences in stability, it is important to consider the stability of each different analyte when it is measured in stored samples and avoid the use of samples stored in conditions in which the analyte is not stable.

EVENTI & NEWS in evidenza

EVENTI & NEWS in evidenza

 CONTATTI

CLINICA VETERINARIA e
 PRONTO SOCCORSO

per informazioni

  • Tel

    049 85 610 98

per appuntamenti o comunicazioni alla segreteria

LABORATORIO D'ANALISI

(riservato ai medici veterinari)

AMMINISTRAZIONE

SPORTELLO PER I MEDICI VETERINARI UTENTI DEL LABORATORIO

Vi invitiamo a utilizzare questo indirizzo anche per suggerimenti ed eventuali reclami relativi all'attività del Laboratorio

AGGIORNAMENTO PER I COLLEGHI – EVENTI SAN MARCO

 DOVE SIAMO

La Clinica/Lab Veterinaria Privata S. Marco è in Via dell'Industria 3 35030 Veggiano (Padova)

Tel. 24 h - 0498561098

Ci trovate lungo la statale Padova-Vicenza, nel comune di Veggiano (tra Mestrino e Grisignano di Zocco), a pochi chilometri dal centro di Padova e dal centro di Vicenza.
Siamo facilmente raggiungibili con l’autostrada A4, a 2Km dall’uscita di Grisignano di Zocco, in direzione Padova.

TORNA SU